The plague is back: The bioweapon of choice

We need a vaccine for the plague, which has frightening potential if it falls into the wrong hands

Lydia Zuraw
Wednesday 11 January 2017 13:51 EST
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The dead and dying in London during the great plague of 1665. A cart in the background carries away those who have already succumbed
The dead and dying in London during the great plague of 1665. A cart in the background carries away those who have already succumbed (Getty)

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The plague is best known for wiping out as much as a third of Europe’s population during the Black Death pandemic of the 14th century, but it’s not entirely a thing of the past. It’s enough of a present-day threat – either as a potential bioterrorism weapon or because some strains are now antibiotic resistant – that scientists are trying to develop a vaccine. Among them are Dr Ashok Chopra and a team of researchers at the University of Texas Medical Branch at Galveston, who recently published a study of the three newest candidates for a plague vaccine in the journal Nature.

“So far, it looks very promising, at least in the two animal models we have tested,” said Chopra, a professor of microbiology and immunology who began studying the bacteria that causes the plague, Yersinia pestis, around 2002.

Residents of the Rhondda Valley queuing in 1962 for their smallpox vaccinations, after the Welsh area was declared to be an infected area
Residents of the Rhondda Valley queuing in 1962 for their smallpox vaccinations, after the Welsh area was declared to be an infected area (Getty)

He began his work following the anthrax attacks of 2001, when letters containing anthrax were mailed to media outlets and congressional offices. Congress required the departments of Health & Human Services and Agriculture to regulate certain biological agents and toxins that could pose a severe threat to public health.

The bacteria responsible for the plague made the top section of the list – Tier 1, the microbes most likely to be used as for bioterrorism agents. It’s listed alongside anthrax, Ebola, smallpox and foot-and-mouth disease.

But 2001 wasn’t the first time the plague was considered a potential bioweapon, said Dr Paul Mead, a medical epidemiologist with the Centres for Disease Control and Prevention. Japan may have spread plague-infected fleas in certain parts of China during the Second World War, and the US and the Soviet Union considered spreading the plague bacteria as an aerosol during the Cold War.

There are three types of plague infection – bubonic, septicemic and pneumonic – and all are caused by the Yersinia pestis bacteria. Left untreated, the bubonic form has a 40 per cent to 70 per cent mortality rate. Pneumonic and septicaemic plague are virtually always fatal.

A Doctors Without Borders (MSF) health worker in protective clothing carries a child suspected of having Ebola in the treatment centre in Paynesville, Liberia
A Doctors Without Borders (MSF) health worker in protective clothing carries a child suspected of having Ebola in the treatment centre in Paynesville, Liberia (Getty)

In the pre-antibiotic era (1900-1941), the mortality among those infected with plague in the US ranged from 66 per cent to 93 per cent. But antibiotics like streptomycin and gentamicin are used to treat all three strains now, so that mortality has been reduced to 11 per cent.

According to the World Health Organisation, there were 783 cases of the plague reported worldwide in 2013, including 126 deaths. It affects people in rural areas in central and southern Africa, central Asia and the Indian subcontinent, the northeastern part of South America and parts of the southwestern US.

The three most heavily affected countries are Madagascar, the Democratic Republic of Congo and Peru. According to the CDC, the US sees an average of seven human plague cases each year. In 2015, there were 16 cases, including two teenagers who visited Yosemite National Park in California. Four of the 16 cases were fatal. As of early November, only four cases had been reported and all patients recovered, according to the CDC.

Vaccines for the plague exist, but they have some serious flaws. One made with dead bacteria is approved by the US Food and Drug Administration but no longer manufactured. It protected against only the bubonic plague, not the more dangerous pneumonic plague.

Another vaccine is used in endemic regions such as China and the former Soviet Union, but it’s not approved by the FDA because of its high likelihood to cause severe side effects such as fever, malaise and headaches.

A brain specimen shows haemorrhagic meningitis caused by inhaling anthrax
A brain specimen shows haemorrhagic meningitis caused by inhaling anthrax (Getty)

The vaccines Chopra reported on in Nature protect against the pneumonic plague and don’t cause side effects. The researchers deleted three genes in each of three candidate strains of plague in order to weaken – but not kill – the bacteria.

The strains could no longer cause disease, but they generated a robust immune response in the animals tested. The team gave two doses of each vaccine to mice and then challenged the animals’ immunity with highly virulent plague strains.

The mice were protected up to four months later. Two of the mutant strains were also successfully tested in rats. Chopra also has a different type of plague vaccine in the works which uses the bacteria’s antigens. In tests in non-human primates, it was shown to be highly protective. “Everybody’s immune system is different, so some people could be protected and some may not be,” Chopra said. “Our goal is to do parallel studies so that we have in our pipeline several vaccine candidates.”

© Newsweek

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